CLA – Caseous Lymphadenitis

Complete genome sequencing and annotation of Corynebacterium pseudotuberculosis isolate

Aim

The aim of this project was to sequence the genome of a challenge strain of Corynebacterium pseudotuberculosis that currently exists in the laboratories of the Moredun Institute. It aimed to provide an essential resource for research into Caseous lymphadenitis (CLA) that could lead to the production of a vaccine for this disease.

Why is it important?

CLA is a chronic, suppurative disease caused by the bacterium Corynebacterium pseudotuberculosis. It can affect a number of domestic species and man but it is as a disease of sheep and goats that it is most important. It occurs worldwide and its presence in a flock creates major welfare and financial problems. Currently the disease is untreatable and there is no licensed vaccine in the UK.

How did the project work?

Deoxy-ribonucleic acid (DNA) was prepared from the strain of C. pseudotuberculosis in the Moredun Institute and used to sequence the genome. This genome was then published to allow its use as a resource in further research to develop control measures for CLA.

Where was the work being done?

The work was carried out at the Moredun Institute in Edinburgh and was being funded by HCC, EBLEX, QMS and LMCNI.

To view the final report, click here